Detailed Mechanism Funding and Narrative

Details for Mechanism ID: 5013
Country/Region: Zambia
Year: 2007
Main Partner: University of Nebraska
Main Partner Program: NA
Organizational Type: University
Funding Agency: HHS/NIH
Total Funding: $280,000

Funding for Laboratory Infrastructure (HLAB): $280,000

This activities is linked to #8887, #8993, ART in Lusaka and the New COAG.

Reliable laboratory support is critical for treatment and care of HIV/AIDS patients. This activity has provided the University Teaching Hospital (UTH) Department of Pediatrics, and the Kalingalinga-Lusaka Health District with training of laboratory personnel and the equipment needed to perform Polymerase Chain Reaction (PCR) diagnosis of HIV-exposed infants, and HIV genotyping for the monitoring of drug resistance. To date six lab technicians have been trained from the two facilities and are now performing PCR and genotyping and about 3,500 tests have been performed including HIV testing, tuberculosis diagnosis, syphilis testing and HIV disease monitoring. Through US Government (USG) funding UTH now has machines and performs PCR and drug monitoring.

In particular, the PCR technique on whole blood or dried blood spots is important for scaling up pediatric treatment in Zambia because it has made it possible to diagnose HIV infection in infants during the first few months of life, as opposed to waiting for 18 months to perform a serological diagnosis. Early infant diagnosis is now enabling early intervention so the infected infants receive specific treatments with antiretroviral therapy and/or other preventive measures such as cotrimoxazole prophylaxis. The Centers for Disease Control and Prevention has placed one full time laboratory technologist with expertise in molecular biology as well as diagnostic laboratory testing assigned to support the activity at the UTH Department of Pediatrics in addition to five technologists in the center. Children on therapy are now monitored for CD4 analysis, full blood count, kidney and liver function testing.. An additional benefit of this activity is the ability to monitor the impact of PMTCT in reducing transmission of infection.

In FY 2007, funds will be used to continue PCR and genotype testing at UTH and Kalingalinga. Technical expertise from this center will support infrastructure development of a second site in the Arthur Davison's Children's Hospital in Ndola. Lessons learned from this activity in FY 2006 will be applied to expand the PCR and genotyping activities to the Arthur Davison's Children's Hospital in Ndola. Additional six staff will be trained in Ndola to perform PCR and HIV genotyping for ARV drug resistance monitoring and about 7000 tests will be performed. Working with the Ministry of Health and provincial health offices and other stakeholders - the University of Nebraska-Lincoln, Health Services and Systems Program, and World Health Organization, UTH will formulate a strategy for conducting baseline for ARV drug resistance monitoring in Zambia.

Initiating and scaling up PCR and ARV drug resistance monitoring at the government hospitals in collaboration with the Ministry of Health is allowing these government institutions to build national capacity through acquiring skills and equipment necessary to scale up and maintain high standard of pediatric ART care. PCR training has been provided to Zambian nationals so that the skills are retained in the country. Under this activity Zambians trained in FY 2006 will work with facilities in other provincial hospitals to transfer their knowledge and skills on PCR and resistance monitoring activities so more children can access treatment as well as build a sustainable pediatric treatment at the provincial levels.